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Setting Up PCR Programs
and Methods 5
Introduction
This chapter describes how to use the HOLD, CYCL, and AUTO
programs to create your own PCR programs. You will learn how
to store, access, edit, and print these programs.
This chapter also describes how to setup a PCR method, in
which you specify a sequence of programs for a run. For
example, pre-PCR incubations, PCR cycling, and post-PCR
incubations can be linked together in a single method. You will
also learn how to store, edit, and print the methods you create.
At the end of this chapter, you will find information on the displays
found in the HOLD, CYCL, and AUTO programs.
5
- PCR System 9600 1
- Part No 2
- Version Date 2
- Back of Instrument 7
- LEFT SIDE 9
- BACK VIEW 10
- United States (FCC) 11
- Operating Precautions 12
- Technical Support 16
- To Contact 17
- Outside North America 18
- To Reach 20
- Through the 20
- Internet 20
- To Obtain 21
- Documents on 21
- Users Manual Summary 23
- Sample Block 26
- Sample Tubes 26
- Comparing Files and Programs 27
- Function Keys 28
- File Storage 29
- Operation 29
- Figure 1 31
- System 9600 31
- If your change in 32
- Subtract this time from your 32
- Thermal Cycler hold time: 32
- Contents 1 35
- Illustrations 11 43
- Preface: Using the Manual P 47
- GeneAmp PCR System 9600: 48
- Conventions 49
- Notes and Warnings 50
- Introducing the GeneAmp 51
- PCR System 9600 1 51
- Principles of Operation 52
- Instrument Description 53
- A Look at the Control Panel 54
- Installing the GeneAmp 57
- PCR System 9600 2 57
- Unpacking 58
- Parts Supplied 58
- within the U.S 59
- Power Requirements 60
- Proper Location 61
- Setting Up the Printer 62
- Figure 2-1 64
- Figure 2-2 65
- User's manual 67
- Time HH:MM 69
- Date MM/DD/YY 69
- Runtime printer OFF 69
- Runtime beeper ON 69
- Heater and Chiller Tests 75
- Tutorial: Customizing a 79
- PCR CYCL Program 3 79
- Before You Start 80
- What is a CYCL Program? 81
- Figure 3-2 82
- Starting Up the Instrument 83
- Getting to the CYCL Program 84
- A Look at the CYCL Program 87
- Setpt #2 Ramp 0:00 88
- 55.0C Hold 0:30 88
- Setpt #3 Ramp 0:00 88
- 72.0C Hold 0:30 88
- To d i s p lay 89
- Figure 3-4 91
- Customizing the CYCL Program 92
- To change NO to 96
- YES and vice versa 96
- Program Type 97
- Storing Your CYCL Program 98
- Assigned Program Number (1) 99
- Starting a Trial Run 100
- Figure 3-5 101
- Ramp to 96.5C xx.xC 103
- 0:30 Cycle 1 103
- 0:22 Cycle 1 103
- Total cycles in program 104
- Current setpoint number 104
- The Next Step 105
- Software Basics 4 107
- Reviewing Keypad Basics 108
- A Close Look at the Main Menu 110
- Figure 4-2 111
- Storing Programs and Methods 113
- The HOLD Program 114
- Figure 4-4 115
- Figure 4-5 115
- CYCL: The Cycle Program 116
- Figure 4-6 117
- A common CYCL program 117
- The AUTO Program 118
- Figure 4-7 119
- PCR Methods 120
- Setting Up PCR Programs 121
- Figure 5-1 123
- PCR System 9600 123
- Figure 5-2 125
- Figure 5-3 127
- Editing Keys 128
- Creating and Editing Programs 129
- Program Type: 130
- Storing the Program 131
- Enter program # x 131
- Protect program? NO 131
- Enter user # 132
- Prog # x User #xxxx 132
- OK to store? YES 132
- Enter Program # x 134
- Prog x is protected 135
- Enter user # _ 135
- Creating and Editing Methods 137
- (Method) 140
- Printing a Method 145
- DATA option 146
- Software Shortcuts 148
- Returns you to the main menu 148
- CYCL#??? xx.xC 149
- UN-STORE-PRINT-HOME 149
- Program Number (26) 150
- Running PCR Samples6 167
- Loading Samples 168
- Figure 6-1 169
- Figure 6-2 170
- Figure 6-3 171
- Figure 6-4 172
- Figure 6-5 173
- Turning the cover knob 173
- Using the Cap-Installing Tool 174
- Figure 6-6 175
- Starting the Run 177
- ! WARNING ! 181
- Figure 6-9 183
- Figure 6-10 184
- Using the MORE key 184
- Figure 6-11 184
- Figure 6-12 185
- Pausing a Run 186
- Stopping a Run 186
- CYCL# 26 Abort xx.xC 187
- History 188
- Utilities 7 189
- Getting to the Utilities Menu 190
- Figure 7-1 191
- The Utilities functions 191
- The Directory Function (DIR) 192
- Directory 194
- Enter program # 1 194
- HOLD #14 196
- HOLD #26 196
- Figure 7-3 197
- CYCL #6 User#7777 199
- HOLD #5 User#7777 199
- Figure 7-4 200
- Directory function 203
- Figure 7-6 205
- The Configuration function 205
- Figure 7-7 214
- Start clock within 215
- C of setpoint 215
- Rxn vol=100uL T= 9.5 217
- Rxn vol= 50uL T= 7.0 217
- Rxn vol= 20uL T= 5.0 217
- Enter test # 224
- Figure 7-8 225
- Figure 7-9 229
- Figure 7-10 232
- Figure 7-11 234
- CYCL program 234
- Figure 7-12 235
- History Print 237
- Heater Test Blk=xx.x 239
- Heater Test Passed 239
- Chiller Test Blk=xx.x 241
- Chiller Test Passed 241
- System Performance 243
- IEW-RUN-PRINT 243
- The Delete Function (DEL) 245
- Delete 246
- Enter program #xxx 246
- Can't delete file xxx 248
- Linked in method xxx! 248
- User #xxxx 248
- Delete all progs?YES 248
- Maintenance 8 251
- Cleaning the Sample Wells 252
- Cleaning the Heated Cover 253
- Equipment Required 254
- Figure 8-1 255
- Select Option 9600 256
- UN-CREATE-EDIT-UTIL 256
- Record Temperature 259
- Time=MM:SS Blk=95.0C 259
- Test Results 260
- Temperature Uniformity Test 263
- Figure 8-5 264
- Figure 8-7 266
- Test Resul ts 267
- Changing External Fuses 268
- Fuse Holders 268
- Troubleshooting 9 269
- (continued) 271
- If the Instrument Turns Off 273
- Specifications 10 275
- Power Requirements 276
- Control Panel 276
- Sample Temperature Range 277
- Sample Temperature Accuracy 277
- Displayed Sample Temperature 277
- Sample Temperature Overshoot 277
- Ramp Time Repeatability 278
- Printer Interface 279
- Temperature Calibration 279
- Index-10 290
- Index-11 291
- Index-12 292
- business 294
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