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4-14
PCR Methods
PCR methods are used to automatically link together up to 17
programs for a specific PCR application. To create a method, you
use the METH program. An example of a method is shown in
Figure 4-8. This method starts with a HOLD file used for pre-PCR
denaturation (e.g., 94.0 degrees C for five minutes). This is
followed by a CYCL program for three-temperature PCR. A final
HOLD program concludes the run, for post-PCR storage
overnight (at 4.0 degrees C, for example).
Figure 4-8
An example of a PCR method.
- PCR System 9600 1
- Part No 2
- Version Date 2
- Back of Instrument 7
- LEFT SIDE 9
- BACK VIEW 10
- United States (FCC) 11
- Operating Precautions 12
- Technical Support 16
- To Contact 17
- Outside North America 18
- To Reach 20
- Through the 20
- Internet 20
- To Obtain 21
- Documents on 21
- Users Manual Summary 23
- Sample Block 26
- Sample Tubes 26
- Comparing Files and Programs 27
- Function Keys 28
- File Storage 29
- Operation 29
- Figure 1 31
- System 9600 31
- If your change in 32
- Subtract this time from your 32
- Thermal Cycler hold time: 32
- Contents 1 35
- Illustrations 11 43
- Preface: Using the Manual P 47
- GeneAmp PCR System 9600: 48
- Conventions 49
- Notes and Warnings 50
- Introducing the GeneAmp 51
- PCR System 9600 1 51
- Principles of Operation 52
- Instrument Description 53
- A Look at the Control Panel 54
- Installing the GeneAmp 57
- PCR System 9600 2 57
- Unpacking 58
- Parts Supplied 58
- within the U.S 59
- Power Requirements 60
- Proper Location 61
- Setting Up the Printer 62
- Figure 2-1 64
- Figure 2-2 65
- User's manual 67
- Time HH:MM 69
- Date MM/DD/YY 69
- Runtime printer OFF 69
- Runtime beeper ON 69
- Heater and Chiller Tests 75
- Tutorial: Customizing a 79
- PCR CYCL Program 3 79
- Before You Start 80
- What is a CYCL Program? 81
- Figure 3-2 82
- Starting Up the Instrument 83
- Getting to the CYCL Program 84
- A Look at the CYCL Program 87
- Setpt #2 Ramp 0:00 88
- 55.0C Hold 0:30 88
- Setpt #3 Ramp 0:00 88
- 72.0C Hold 0:30 88
- To d i s p lay 89
- Figure 3-4 91
- Customizing the CYCL Program 92
- To change NO to 96
- YES and vice versa 96
- Program Type 97
- Storing Your CYCL Program 98
- Assigned Program Number (1) 99
- Starting a Trial Run 100
- Figure 3-5 101
- Ramp to 96.5C xx.xC 103
- 0:30 Cycle 1 103
- 0:22 Cycle 1 103
- Total cycles in program 104
- Current setpoint number 104
- The Next Step 105
- Software Basics 4 107
- Reviewing Keypad Basics 108
- A Close Look at the Main Menu 110
- Figure 4-2 111
- Storing Programs and Methods 113
- The HOLD Program 114
- Figure 4-4 115
- Figure 4-5 115
- CYCL: The Cycle Program 116
- Figure 4-6 117
- A common CYCL program 117
- The AUTO Program 118
- Figure 4-7 119
- PCR Methods 120
- Setting Up PCR Programs 121
- Figure 5-1 123
- PCR System 9600 123
- Figure 5-2 125
- Figure 5-3 127
- Editing Keys 128
- Creating and Editing Programs 129
- Program Type: 130
- Storing the Program 131
- Enter program # x 131
- Protect program? NO 131
- Enter user # 132
- Prog # x User #xxxx 132
- OK to store? YES 132
- Enter Program # x 134
- Prog x is protected 135
- Enter user # _ 135
- Creating and Editing Methods 137
- (Method) 140
- Printing a Method 145
- DATA option 146
- Software Shortcuts 148
- Returns you to the main menu 148
- CYCL#??? xx.xC 149
- UN-STORE-PRINT-HOME 149
- Program Number (26) 150
- Running PCR Samples6 167
- Loading Samples 168
- Figure 6-1 169
- Figure 6-2 170
- Figure 6-3 171
- Figure 6-4 172
- Figure 6-5 173
- Turning the cover knob 173
- Using the Cap-Installing Tool 174
- Figure 6-6 175
- Starting the Run 177
- ! WARNING ! 181
- Figure 6-9 183
- Figure 6-10 184
- Using the MORE key 184
- Figure 6-11 184
- Figure 6-12 185
- Pausing a Run 186
- Stopping a Run 186
- CYCL# 26 Abort xx.xC 187
- History 188
- Utilities 7 189
- Getting to the Utilities Menu 190
- Figure 7-1 191
- The Utilities functions 191
- The Directory Function (DIR) 192
- Directory 194
- Enter program # 1 194
- HOLD #14 196
- HOLD #26 196
- Figure 7-3 197
- CYCL #6 User#7777 199
- HOLD #5 User#7777 199
- Figure 7-4 200
- Directory function 203
- Figure 7-6 205
- The Configuration function 205
- Figure 7-7 214
- Start clock within 215
- C of setpoint 215
- Rxn vol=100uL T= 9.5 217
- Rxn vol= 50uL T= 7.0 217
- Rxn vol= 20uL T= 5.0 217
- Enter test # 224
- Figure 7-8 225
- Figure 7-9 229
- Figure 7-10 232
- Figure 7-11 234
- CYCL program 234
- Figure 7-12 235
- History Print 237
- Heater Test Blk=xx.x 239
- Heater Test Passed 239
- Chiller Test Blk=xx.x 241
- Chiller Test Passed 241
- System Performance 243
- IEW-RUN-PRINT 243
- The Delete Function (DEL) 245
- Delete 246
- Enter program #xxx 246
- Can't delete file xxx 248
- Linked in method xxx! 248
- User #xxxx 248
- Delete all progs?YES 248
- Maintenance 8 251
- Cleaning the Sample Wells 252
- Cleaning the Heated Cover 253
- Equipment Required 254
- Figure 8-1 255
- Select Option 9600 256
- UN-CREATE-EDIT-UTIL 256
- Record Temperature 259
- Time=MM:SS Blk=95.0C 259
- Test Results 260
- Temperature Uniformity Test 263
- Figure 8-5 264
- Figure 8-7 266
- Test Resul ts 267
- Changing External Fuses 268
- Fuse Holders 268
- Troubleshooting 9 269
- (continued) 271
- If the Instrument Turns Off 273
- Specifications 10 275
- Power Requirements 276
- Control Panel 276
- Sample Temperature Range 277
- Sample Temperature Accuracy 277
- Displayed Sample Temperature 277
- Sample Temperature Overshoot 277
- Ramp Time Repeatability 278
- Printer Interface 279
- Temperature Calibration 279
- Index-10 290
- Index-11 291
- Index-12 292
- business 294
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